LASER ASSISTED HATCHING (LAH):
The ability of the embryo to hatch from its protective shell is a crucial step in enabling it to implant in the uterus.
What are the functions of laser assisted hatching?
The egg's shell (zona pellucida) has a number of functions, the last of which is
1) To hatch or free the growing embryo so that it may implant.
2) To create a barrier so that no more than one sperm fertilizes the egg as p... Read More
The natural process of embryo development begins with the fertilization of the egg in the outer aspect of the fallopian tube. As the newly formed embryo develops, it moves slowly toward the uterine cavity where it will ultimately implant. This process takes approximately six to seven days.
What is Blastocyst?
5th day embryo is known as Blastocyst, where it is ready to hatch and implant.
Wha... Read More
What is eggfreezing?
Freezing / cryo preservation of eggs is process in which the oocytes are treated with cryoprotective substance and submerged in liquid nitrogen (-196degree C).
Why is eggcryopreservation done?
Oocyte cryopreservation via vitrification is one of the techniques used in preserving a woman’s fertility when she is young and the eggs are of higher qualitytherefore allowing reproductive capacity to be postponed unti... Read More
What is embryo freezing / cryopreservation?
Embryo cryopreservation is the process of preserving an embryo obtained from IVF /ICSI procedure that is, from fertilization to cleavage stage(day3) or blastocyst stage(day5) at sub-zero temperature (-196degreeC) using Vitrificationtechnique. Embryo freezing is a great way to preserve your fertility. Vitrification appears to result in decreased risk of DNA damage than any other freezing technique.... Read More
Intracytoplasmic Morphologically Selected Sperm Injection (IMSI)
is an advanced assisted reproductive technology done in cases of severe male factor infertility.
What is IMSI?
IMSI is an advanced version of ICSI having a magnification power of 16 times higher compared to ICSI.In ICSI, before sperm microinjection the sperms are observed under the microscope of x500 time’s magnification. But in IMSI, the sperm is being examine... Read More
Preimplantation Genetic Testing for Aneuploidy (PGT-A)
Chromosomes are genetic structures found in nearly every cell of our bodies. The first cell of an embryo is made up of a set of chromosomes from the sperm and another set from the egg. But if the cell doesn’t have the right number of chromosomes, known as aneuploidy, this can lead to implantation failure, miscarriage or a baby affected with a serious condition.
PGT-A is a technique that allows s... Read More
What is PICSI?
The PICSI(Physiological Intracytoplasmic Sperm Injection) procedure is the actual dish with the hyaluronan enzyme micro dots. Hyaluronan is a naturally occurring biopolymer found in all human cells including the gel layer surrounding the oocyte.The hyaluronan enzyme is the agent that acts like a “magnet” to attract the healthiest and matured sperm. This enzyme allows the sperm to bind to these micro dots on the dish.
... Read More
Sperm freezing is the process of collecting, analyzing, freezing and storing of sperms. The samples are later used for fertility treatments. This overall process is known as cryopreservation and is sometimes referred to as sperm banking. Semen freezing is also done as part of fertility preservation too.
HOW IS SEMEN FREEZING DONE?
Semen sample is first checked for the count and motility... Read More
SPERM PREPARATION METHODS
Semen is a mixture of motile, non-motile and dead spermatozoa with cells, cellular debris and sometimes micro-organisms too.
There are various sperm separation and isolation methods. These methods try to replicate in vitro the natural process in which viable sperm are separated from other constituents of the ejaculate as they actively migrate through the cervical mucus.
Semen sample processing should be... Read More
SPERM DNA FRAGMENTATION TEST
Fertilization and subsequent embryo development depend in part on the inherent integrity of sperm DNA. Sperm DNA is bound to protamine and is naturally present in a compact state, protecting it from damage during transport. Some damage can occur, which can be repaired in the cytoplasm of the oocyte. When the damage exceeds the cytoplasm’s repair threshold, however, infertility can ensue.
A conventional semen analysis do... Read More