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Embryo Freezing

What is embryo freezing / cryopreservation?

Embryo cryopreservation is the process of preserving an embryo obtained from IVF /ICSI procedure that is, from fertilization to cleavage stage(day3) or blastocyst stage(day5) at sub-zero temperature (-196degreeC) using Vitrification technique. Embryo freezing is a great way to preserve your fertility. Vitrification appears to result in decreased risk of DNA damage than any other freezing technique.

Why is embryo freezing done?

Embryo cryopreservation is done

  • If the woman is responding excessively to the stimulation drugs and she has produced too many eggs, she will be at risk of developing ovarian hyperstimulation syndrome (OHSS) if embryos were transferred in the same cycle.
  • Endometrial problems: Thin endometrium, endometrial polyp, Fluid in the endometrium, Thick endometrium. These problems will affect the lining of the womb making it less receptive to embryos.
  • The woman may have other medical condition that requires treatment before transferring the embryos back to the womb.
  • Asynchrony of the treatment cycle between donor and recipient / surrogate.
  • Excessive embryos remaining after embryo transfer during IVF treatment, where it could be utilised if failed or for the second pregnancy.
  • Cryopreservation of the extra embryos to avoid multiple pregnancies.
  • It allows Preimplantation genetic screening (PGS) that tests looking for chromosomal abnormalities.


How is embryo freezing done?

Embryo obtained out of IVF/ICSI procedure on day3 or day5 of culture are frozen using vitrification technique which is an advanced method.

Embryos are put in embryo containers called storage straws and then frozen by vitrification (rapid freezing). The straws containing the embryos are eventually stored in liquid nitrogen tanks. Water in the cell is replaced by a substance known as cryoprotectant. Embryos are further left to equilibrate in the cryoprotectant increasing levels before the freezing starts. The embryos are frozen in their preservation stage after this.

The cryoprotected embryos freeze in this procedure so quickly that the molecules of water do not get enough time to form crystals of ice. The embryos are protected and their chances of survival increase during thawing.

The vitrification method has a higher embryo survival rate on thawing as compared to the old-fashioned slow freezing method, and has therefore become the preferred method of freezing embryos nowadays.

Is there any chance where the embryos are mixed up?

NO! All Straws or ampoules used for each patient’s embryos are carefully and permanently labeled for later identification purpose.

Will long storage period have impact on embryo?

The outcome from using long period cryopreserved embryos has uniformly been positive with no increase in birth defects or any other abnormalities. Your chances of becoming pregnant with a thawed frozen embryo are not affected by the length of time the embryo has been stored for.

How long can the embryo be stored?

In India, as per ICMR guidelines, embryos can be frozen for a maximum period of five years. If patient wish to extend this period, they are requested to inform the clinic in prior.

Do the couple / patient need to pay for embryo freezingto extend for certain period of time?

Constant maintenance of embryo freezing need frequent use of liquid nitrogen to fill the cryo tank. Fixed charges should be paid periodically once in every 6 months or every 12 months until they are transferred or discarded.

What is the survival rate of frozen embryos when thawed in the future? 

Survival of the embryos depends upon the quality of the embryos at the timing of freezing. Embryos are more stable and with the new vitrification technique, survival is more than 90-95% after thawing.

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What Our Client Say

We love that our patients feel inspired to write about the care they received here at Vishvas Fertility! Here are some of the wonderful letters and comments we have recently received.

pramod kr
pramod kr
We visited Dr. Basavaraj for our treatment. We had to visit for over an year and couple of procedures. Doctor is very knowledgeable and has vast experience. Nurses and other lab staff are good. Initially the wait time was less ,however off kate the wait time is on higher side since doctor does scan and procedures in parallel. The wait time doesn't change even with prior appointment . This is especially true in Marathahalli centre. This can be improved. I feel cost here for treatment is much lesser than any corporate hospitals or other centres. Doctor is mostly straight forward and not verbose. Also doctor prescribes just enough medication which might or might not be good, hard to judge.
Rajeev Kothamachu
Rajeev Kothamachu
I highly recommend the doctor if there any complications. I visited him and we got very good results. He has very good patience and listen all the problems. Doc is not that commercial he is collecting the same from last 4 years. We are now visiting him again for 2nd pregnancy..
Divya Sharma
Divya Sharma
Dr Basavaraj is amazing and genuine person. Report asked by him are only needed one, no additional like many other place. All clinic staff are even good n helpful, Appointment recommended Over all very good Dr for fertility.
Vinay K
Vinay K
Very good doctor , provides good information about the procedures ,
Abhijit Sarkar
Abhijit Sarkar
We worship God and many says that Doctors are possibly the one. We (me and my wife) evident that in our difficult time. Thank you Dr. Basavraj for your treatment and care. You will always be there in our heart and any word of gratitude is not enough. We have blessed with a baby boy and he is doing great. we were struggling from almost 3 years and finally we have been blessed. We (me and my wife) can never forgot what you did for us. When in difficult time people goes away, you have been our hope and belief. we were really very lucky enough that we got a chance to meet you in person and got the treatment. Our Best wishes to you Doctor and may you help many more people who are suffering from fertility issues. We were really amazed that even after such a busy schedule doctor finds the time to respond whenever it was required (in panic situations). Thank you once again doctor for your personal attention. Our best wishes for you and your family. We will surely come and meet you very soon, I am sure my son also will be blessed by just seeing you. You have given us the reason to Smile 🙂
Bharath Konegadde
Bharath Konegadde
Dr Basavaraj Shetty take care and advice till 3 months pregnancy . One of the best doctor in hsr
Dr Chetan Kumar N.G
Dr Chetan Kumar N.G
Dr Devarshetty has a midas touch in his treatment apart from his knowledge skills as a infertility specialist. I recommend all patients to be open minded while consulting him. My wife had a very difficult phase in conceiving after getting ectopic pregnancy twice in 2015-16 because of her PCOD . Being a Dr myself i couldn't cope up with the stress with which i and my wife underwent during those times,later in 2016-17 after Dr Devarshetty advise and treatment we finally became parents and it was the most joyous moment of our married life. Now we have a healthy baby girl 10m old..posted mother and baby image.