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Semen Freezing


Sperm freezing is the process of collecting, analyzing, freezing and storing of sperms. The samples are later used for fertility treatments. This overall process is known as cryopreservation and is sometimes referred to as sperm banking. Semen freezing is also done as part of fertility preservation too.


Semen sample is first checked for the count and motility. Man has to produce a fresh sperm sample on the day of planned sperm freezing. The sample is mixed with a special fluid called a cryoprotectant, to protect the sperm from damage during the freezing process.

The sample is then divided between a number of containers called cryotubes depending upon the volume of the raw sample, so that each cryo tube can be thawed independently and the stored sperm can be used in multiple fertility treatments in the future. The cryotubes containing the sperm are then plunged into liquid nitrogen tanks for storage at -196o C till later used in IUI, IVF or ICSI treatment

Will there be any damage to the frozensperms due to semen freezing and thawing?

In frozen semen sample, there may be 20%, but not significant reduction in the sperm motility after thawingit.

Who all can benefit and what are the indications for semen sample freezing?

Sperm cryopreservation for male fertility preservation is offered for the following cases:

  • When there is a risk of reproductive ability loss (testis removal, chemotherapy, radiotherapy.)
  • When the male partner will not be in station on the day of oocyte retrieval.
  • When the male partner has difficulty in providing semen sample on demand
  • In cases of surgical sperm recovery.
  • In cases of sperm recovery using electro ejaculation.
  • In cases of ejaculation problems.
  • In cases of progressively declining sperm quality.
  • Before sterilization by vasectomy.
  • Donor semen sample programme

What are the instructions to be given or prerequisites prior to semen sample freezing?

  • Should be tested for infectious diseases including HIV, Syphilis, Hepatitis B and Hepatitis C within 6 months of providing semen sample.
  • The man should have abstinence of 2-3 days prior to the day of providing semen sample.
  • A written consent form is taken prior

How many sperm samples to freeze?

Sperm may not be able to maintain their viability and mobility during the freezing and thawing processes. In general, around 50% – 80% of the sperm can survive after thawing, and the percentage is even lower if the quality of the pre-freeze sample is already not the best.

For men who are freezing the sperm as a backup storage for fertility treatment, one sample is usually adequate.

For men who are freezing the sperm for fertility preservation because of a medical condition, the underlying disease may have caused a reduction in the sperm count and motility. In such situations, it may be advisable to freeze more than one sample if possible, preferably between two to five, depending on the time interval before surgery, chemotherapy or radiotherapy, so that there will be adequate sperm for use in the future after thawing.

What happens when a man wants to use his sperm?

When the man is ready to use his sperm, the sperm are thawed and used in fertility treatments. The type of treatment depends on the number of cryotubes in storage and the sperm quality. Frozen sperm can be used for intrauterine insemination (IUI) if the sperm quality is good. If the sperm quality is poor, it may be necessary to undergo in-vitro fertilization (IVF) or even intracytoplasmic sperm injection (ICSI) whereby a single sperm is injected directly into an egg to assist fertilization.

How long can the sperm are stored for?

Currently if a man is storing sperm for social reasons, the sperm can only remain in storage for up to a maximum of 5 years.

Can own sperm be donated if decided not to use it further?

Own frozen semen sample cannot be donated. It can be discarded or used for teaching purpose.

Do you need to pay if you want to continue semen freezing for future use?

Constant maintenance of semen freezing need frequent use of liquid nitrogen to fill the cryo tank. Fixed charges should be paid periodically once in every 6 months or every 12 months.

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What Our Client Say

We love that our patients feel inspired to write about the care they received here at Vishvas Fertility! Here are some of the wonderful letters and comments we have recently received.

pramod kr
pramod kr
We visited Dr. Basavaraj for our treatment. We had to visit for over an year and couple of procedures. Doctor is very knowledgeable and has vast experience. Nurses and other lab staff are good. Initially the wait time was less ,however off kate the wait time is on higher side since doctor does scan and procedures in parallel. The wait time doesn't change even with prior appointment . This is especially true in Marathahalli centre. This can be improved. I feel cost here for treatment is much lesser than any corporate hospitals or other centres. Doctor is mostly straight forward and not verbose. Also doctor prescribes just enough medication which might or might not be good, hard to judge.
Rajeev Kothamachu
Rajeev Kothamachu
I highly recommend the doctor if there any complications. I visited him and we got very good results. He has very good patience and listen all the problems. Doc is not that commercial he is collecting the same from last 4 years. We are now visiting him again for 2nd pregnancy..
Divya Sharma
Divya Sharma
Dr Basavaraj is amazing and genuine person. Report asked by him are only needed one, no additional like many other place. All clinic staff are even good n helpful, Appointment recommended Over all very good Dr for fertility.
Vinay K
Vinay K
Very good doctor , provides good information about the procedures ,
Abhijit Sarkar
Abhijit Sarkar
We worship God and many says that Doctors are possibly the one. We (me and my wife) evident that in our difficult time. Thank you Dr. Basavraj for your treatment and care. You will always be there in our heart and any word of gratitude is not enough. We have blessed with a baby boy and he is doing great. we were struggling from almost 3 years and finally we have been blessed. We (me and my wife) can never forgot what you did for us. When in difficult time people goes away, you have been our hope and belief. we were really very lucky enough that we got a chance to meet you in person and got the treatment. Our Best wishes to you Doctor and may you help many more people who are suffering from fertility issues. We were really amazed that even after such a busy schedule doctor finds the time to respond whenever it was required (in panic situations). Thank you once again doctor for your personal attention. Our best wishes for you and your family. We will surely come and meet you very soon, I am sure my son also will be blessed by just seeing you. You have given us the reason to Smile 🙂
Bharath Konegadde
Bharath Konegadde
Dr Basavaraj Shetty take care and advice till 3 months pregnancy . One of the best doctor in hsr
Dr Chetan Kumar N.G
Dr Chetan Kumar N.G
Dr Devarshetty has a midas touch in his treatment apart from his knowledge skills as a infertility specialist. I recommend all patients to be open minded while consulting him. My wife had a very difficult phase in conceiving after getting ectopic pregnancy twice in 2015-16 because of her PCOD . Being a Dr myself i couldn't cope up with the stress with which i and my wife underwent during those times,later in 2016-17 after Dr Devarshetty advise and treatment we finally became parents and it was the most joyous moment of our married life. Now we have a healthy baby girl 10m old..posted mother and baby image.